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precut nitrocellulose filter paper sandwiches  (Bio-Rad)


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    Structured Review

    Bio-Rad precut nitrocellulose filter paper sandwiches
    Precut Nitrocellulose Filter Paper Sandwiches, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/precut+nitrocellulose+filter+paper+sandwiches/Nitrocellulose%2FFilter+Paper+Sandwiches/pmc07100051-133-101-105
    Average 93 stars, based on 176 article reviews
    precut nitrocellulose filter paper sandwiches - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: Purified miRNA (1,000 ng) was converted to cDNA using miScript cDNA synthesis kit (Qiagen) following the manufacturer’s protocol. qRT-PCR was performed with miRNA-specific primers (Qiagen) using miScript SYBR Green PCR master mix (Qiagen) and CFX96 thermal cycler (Bio-Rad). .. Ct values for the selected gene were normalized against RNU6–2 (internal miR expression control) values in the same sample. table ft1 table-wrap mode="anchored" t5 Table caption a7 Target Species Sequence 5′–3′ MCP-1 F Mus musculus GTAGCAGCAGGTGAGTGGGGC MCP-1 R Mus musculus CACAGTTGCCGGCTGGAGCAT IL-1β F Mus musculus CCTCGGCCAAGACAGGTCGC IL-1β R Mus musculus TGCCCATCAGAGGCAAGGAGGA 18SF Mus musculus TTCGAACGTCTGCCCTATCAA 18SR Mus musculus ATGGTAGGCACGGCGATA CD68F Mus musculus AGGGTGGAAGAAAGGTAAAGC CD68R Mus musculus AGAGCAGGTCAAGGTGAACAG F4/80F Mus musculus TTTCCTCGCCTGCTTCTTC F4/80R Mus musculus CCCCGTCTCTGTATTCAACC Open in a separate window showing the primer sequences for the different targets genes: Western blot SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    Control:

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: Purified miRNA (1,000 ng) was converted to cDNA using miScript cDNA synthesis kit (Qiagen) following the manufacturer’s protocol. qRT-PCR was performed with miRNA-specific primers (Qiagen) using miScript SYBR Green PCR master mix (Qiagen) and CFX96 thermal cycler (Bio-Rad). .. Ct values for the selected gene were normalized against RNU6–2 (internal miR expression control) values in the same sample. table ft1 table-wrap mode="anchored" t5 Table caption a7 Target Species Sequence 5′–3′ MCP-1 F Mus musculus GTAGCAGCAGGTGAGTGGGGC MCP-1 R Mus musculus CACAGTTGCCGGCTGGAGCAT IL-1β F Mus musculus CCTCGGCCAAGACAGGTCGC IL-1β R Mus musculus TGCCCATCAGAGGCAAGGAGGA 18SF Mus musculus TTCGAACGTCTGCCCTATCAA 18SR Mus musculus ATGGTAGGCACGGCGATA CD68F Mus musculus AGGGTGGAAGAAAGGTAAAGC CD68R Mus musculus AGAGCAGGTCAAGGTGAACAG F4/80F Mus musculus TTTCCTCGCCTGCTTCTTC F4/80R Mus musculus CCCCGTCTCTGTATTCAACC Open in a separate window showing the primer sequences for the different targets genes: Western blot SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    Sequencing:

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: Purified miRNA (1,000 ng) was converted to cDNA using miScript cDNA synthesis kit (Qiagen) following the manufacturer’s protocol. qRT-PCR was performed with miRNA-specific primers (Qiagen) using miScript SYBR Green PCR master mix (Qiagen) and CFX96 thermal cycler (Bio-Rad). .. Ct values for the selected gene were normalized against RNU6–2 (internal miR expression control) values in the same sample. table ft1 table-wrap mode="anchored" t5 Table caption a7 Target Species Sequence 5′–3′ MCP-1 F Mus musculus GTAGCAGCAGGTGAGTGGGGC MCP-1 R Mus musculus CACAGTTGCCGGCTGGAGCAT IL-1β F Mus musculus CCTCGGCCAAGACAGGTCGC IL-1β R Mus musculus TGCCCATCAGAGGCAAGGAGGA 18SF Mus musculus TTCGAACGTCTGCCCTATCAA 18SR Mus musculus ATGGTAGGCACGGCGATA CD68F Mus musculus AGGGTGGAAGAAAGGTAAAGC CD68R Mus musculus AGAGCAGGTCAAGGTGAACAG F4/80F Mus musculus TTTCCTCGCCTGCTTCTTC F4/80R Mus musculus CCCCGTCTCTGTATTCAACC Open in a separate window showing the primer sequences for the different targets genes: Western blot SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    Western Blot:

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: Purified miRNA (1,000 ng) was converted to cDNA using miScript cDNA synthesis kit (Qiagen) following the manufacturer’s protocol. qRT-PCR was performed with miRNA-specific primers (Qiagen) using miScript SYBR Green PCR master mix (Qiagen) and CFX96 thermal cycler (Bio-Rad). .. Ct values for the selected gene were normalized against RNU6–2 (internal miR expression control) values in the same sample. table ft1 table-wrap mode="anchored" t5 Table caption a7 Target Species Sequence 5′–3′ MCP-1 F Mus musculus GTAGCAGCAGGTGAGTGGGGC MCP-1 R Mus musculus CACAGTTGCCGGCTGGAGCAT IL-1β F Mus musculus CCTCGGCCAAGACAGGTCGC IL-1β R Mus musculus TGCCCATCAGAGGCAAGGAGGA 18SF Mus musculus TTCGAACGTCTGCCCTATCAA 18SR Mus musculus ATGGTAGGCACGGCGATA CD68F Mus musculus AGGGTGGAAGAAAGGTAAAGC CD68R Mus musculus AGAGCAGGTCAAGGTGAACAG F4/80F Mus musculus TTTCCTCGCCTGCTTCTTC F4/80R Mus musculus CCCCGTCTCTGTATTCAACC Open in a separate window showing the primer sequences for the different targets genes: Western blot SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    Polyacrylamide Gel Electrophoresis:

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: Purified miRNA (1,000 ng) was converted to cDNA using miScript cDNA synthesis kit (Qiagen) following the manufacturer’s protocol. qRT-PCR was performed with miRNA-specific primers (Qiagen) using miScript SYBR Green PCR master mix (Qiagen) and CFX96 thermal cycler (Bio-Rad). .. Ct values for the selected gene were normalized against RNU6–2 (internal miR expression control) values in the same sample. table ft1 table-wrap mode="anchored" t5 Table caption a7 Target Species Sequence 5′–3′ MCP-1 F Mus musculus GTAGCAGCAGGTGAGTGGGGC MCP-1 R Mus musculus CACAGTTGCCGGCTGGAGCAT IL-1β F Mus musculus CCTCGGCCAAGACAGGTCGC IL-1β R Mus musculus TGCCCATCAGAGGCAAGGAGGA 18SF Mus musculus TTCGAACGTCTGCCCTATCAA 18SR Mus musculus ATGGTAGGCACGGCGATA CD68F Mus musculus AGGGTGGAAGAAAGGTAAAGC CD68R Mus musculus AGAGCAGGTCAAGGTGAACAG F4/80F Mus musculus TTTCCTCGCCTGCTTCTTC F4/80R Mus musculus CCCCGTCTCTGTATTCAACC Open in a separate window showing the primer sequences for the different targets genes: Western blot SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: .. SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    Membrane:

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: Purified miRNA (1,000 ng) was converted to cDNA using miScript cDNA synthesis kit (Qiagen) following the manufacturer’s protocol. qRT-PCR was performed with miRNA-specific primers (Qiagen) using miScript SYBR Green PCR master mix (Qiagen) and CFX96 thermal cycler (Bio-Rad). .. Ct values for the selected gene were normalized against RNU6–2 (internal miR expression control) values in the same sample. table ft1 table-wrap mode="anchored" t5 Table caption a7 Target Species Sequence 5′–3′ MCP-1 F Mus musculus GTAGCAGCAGGTGAGTGGGGC MCP-1 R Mus musculus CACAGTTGCCGGCTGGAGCAT IL-1β F Mus musculus CCTCGGCCAAGACAGGTCGC IL-1β R Mus musculus TGCCCATCAGAGGCAAGGAGGA 18SF Mus musculus TTCGAACGTCTGCCCTATCAA 18SR Mus musculus ATGGTAGGCACGGCGATA CD68F Mus musculus AGGGTGGAAGAAAGGTAAAGC CD68R Mus musculus AGAGCAGGTCAAGGTGAACAG F4/80F Mus musculus TTTCCTCGCCTGCTTCTTC F4/80R Mus musculus CCCCGTCTCTGTATTCAACC Open in a separate window showing the primer sequences for the different targets genes: Western blot SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: .. SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    Molecular Weight:

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: Purified miRNA (1,000 ng) was converted to cDNA using miScript cDNA synthesis kit (Qiagen) following the manufacturer’s protocol. qRT-PCR was performed with miRNA-specific primers (Qiagen) using miScript SYBR Green PCR master mix (Qiagen) and CFX96 thermal cycler (Bio-Rad). .. Ct values for the selected gene were normalized against RNU6–2 (internal miR expression control) values in the same sample. table ft1 table-wrap mode="anchored" t5 Table caption a7 Target Species Sequence 5′–3′ MCP-1 F Mus musculus GTAGCAGCAGGTGAGTGGGGC MCP-1 R Mus musculus CACAGTTGCCGGCTGGAGCAT IL-1β F Mus musculus CCTCGGCCAAGACAGGTCGC IL-1β R Mus musculus TGCCCATCAGAGGCAAGGAGGA 18SF Mus musculus TTCGAACGTCTGCCCTATCAA 18SR Mus musculus ATGGTAGGCACGGCGATA CD68F Mus musculus AGGGTGGAAGAAAGGTAAAGC CD68R Mus musculus AGAGCAGGTCAAGGTGAACAG F4/80F Mus musculus TTTCCTCGCCTGCTTCTTC F4/80R Mus musculus CCCCGTCTCTGTATTCAACC Open in a separate window showing the primer sequences for the different targets genes: Western blot SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: .. SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    High Molecular Weight:

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: Purified miRNA (1,000 ng) was converted to cDNA using miScript cDNA synthesis kit (Qiagen) following the manufacturer’s protocol. qRT-PCR was performed with miRNA-specific primers (Qiagen) using miScript SYBR Green PCR master mix (Qiagen) and CFX96 thermal cycler (Bio-Rad). .. Ct values for the selected gene were normalized against RNU6–2 (internal miR expression control) values in the same sample. table ft1 table-wrap mode="anchored" t5 Table caption a7 Target Species Sequence 5′–3′ MCP-1 F Mus musculus GTAGCAGCAGGTGAGTGGGGC MCP-1 R Mus musculus CACAGTTGCCGGCTGGAGCAT IL-1β F Mus musculus CCTCGGCCAAGACAGGTCGC IL-1β R Mus musculus TGCCCATCAGAGGCAAGGAGGA 18SF Mus musculus TTCGAACGTCTGCCCTATCAA 18SR Mus musculus ATGGTAGGCACGGCGATA CD68F Mus musculus AGGGTGGAAGAAAGGTAAAGC CD68R Mus musculus AGAGCAGGTCAAGGTGAACAG F4/80F Mus musculus TTTCCTCGCCTGCTTCTTC F4/80R Mus musculus CCCCGTCTCTGTATTCAACC Open in a separate window showing the primer sequences for the different targets genes: Western blot SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.

    Article Title: MICROCYSTIN EXPOSURE WORSENS NONALCOHOLIC FATTY LIVER DISEASE ASSOCIATED ECTOPIC GLOMERULAR TOXICITY VIA NOX-2-MIR21 AXIS
    Article Snippet: .. SDS PAGE-Resolved protein bands were transferred to nitrocellulose membrane using precut nitrocellulose/filter paper sandwiches (Bio-Rad Laboratories, Hercules, CA) and Trans-Blot Turbo transfer system (Bio-Rad) in case of low molecular weight proteins and using wet transfer module from Invitrogen for high molecular weight proteins. .. The membrane was incubated with the primary antibody for PTEN and β-actin (Santa Cruz Biotech.) at dilutions (1:1000), which was then incubated with species-specific horseradish peroxidase-conjugated secondary antibodies (1:5000 dilution) for 1.5 hours.



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